Over the past decade, thousands of non-coding RNAs (ncRNAs) have been observed. MicroRNAs (miRNAs) are a class of ncRNAs consisting of 18-24 nucleotides that are usually highly conserved among different species. Accumulating evidence indicates that miRNAs play important roles in various biological processes such as development, differentiation, and stemness by binding to the 5'UTR, ORF, or 3'UTR of target gene mRNAs to repress their translation or induce their degradation effect.
What Is Human miRNA?
Accumulating evidence indicates that miRNAs are involved in the pathogenesis of many diseases, such as cancer, diabetes, cardiovascular disease and neurodegenerative diseases. Currently, the miRNA repository miRBase lists 1917 human precursor miRNAs (pMIRs) and 2654 mature miRNAs (mMIRs), and it is estimated that more than 60% of human protein-coding genes contain predicted miRNA target sites. Many therapeutic miRNAs and small interfering RNAs (siRNAs) have important biopharmaceutical properties and have been commercially developed as potential medical treatments. Furthermore, the development of reagents that can strongly inhibit and overexpress specific miRNAs is of great interest and is important for basic miRNA research and as a possible therapeutic strategy.
Applications of Human miRNA Cell Line?
The use of human miRNA-overexpressing cell lines is critical for understanding the roles of microRNAs in various biological processes. miRNA-overexpressing cell lines have become an indispensable tool for studying the functional significance of miRNAs in various biological processes. Their ability to promote the stable overexpression of specific miRNAs facilitates the study of their effects on cellular processes. The use of miRNA-overexpressing cell lines has revealed important roles for miRNAs in cancer, neurology, stem cell differentiation, immune response, and cardiovascular disease. The continued development of miRNA-overexpressing cell lines promises to further elucidate the roles of miRNAs in physiology and pathology and may lead to the development of new therapeutic strategies for a range of diseases.
Our Human miRNA Overexpression Stable Cell Lines
miRNA overexpressing cell lines are usually constructed by transfecting a plasmid or viral vector containing the miRNA of interest into host cells. The transfection process promotes the integration of miRNAs into the host genome, resulting in stable overexpression of miRNAs. Creative Biogene is developing stable cell lines overexpressing human miRNA precursors covered in the current miRbase. Our human miRNA overexpression stable cell lines are constructed by lentivirus transduction or non-viral plasmid transfection of vectors optimized by our scientists to enable expression and maturation of miRNAs inside cells.