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Creative Biogene is one of the leading biotechnology companies which has extensive expertise and experience in providing custom vector construction and modification service for customers worldwide. Creative Biogene's sophisticated equipments, advanced technologies and highly experienced staffs are available to provide you with the complete custom vector construction and modification service, including sequences analysis, gene synthesis & cloning, DNA fragment assembling, DNA sequencing, site directed mutagenesis, and plasmid production, etc. Using fast and efficient construction and modification methods, Creative Biogene can quickly create novel custom vector constructs for large or small projects.

In molecular cloning, a vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell, where it can be replicated and/or expressed (e.g.- plasmid, cosmid, Lambda phages). The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes. Among these, the most commonly used vectors are plasmids. Nowadays, a wide variety of plasmid vectors are commercially available. However, in some cases, the commercial vectors cannot best match researcher's specific needs and researchers have to rely on in house vector modification.

With years of development, Creative Biogene is able to provide custom vector construction and modification service to generate custom specialized plasmid vectors with distinct characteristics quickly and reliably. Creative Biogene has developed an advanced gene synthesis platform to modular construct custom plasmid vectors. We obtain the vector modules either by PCR amplification or synthetical methods and using complex cloning strategies when needed to develop completely customized vectors to best fit your unique requirements. Creative Biogene's goal is to provide you with the most affordable, high-quality custom vector construction and modification service to ensure your satisfaction in a timely and professional manner.

Creative Biogene provides a range of popular vector components that you can choose from when designing your specialized vectors.

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Recombinant adenoviruses are efficient tools for gene delivery in mammalian cells, widely used in both in vivo and in vitro applications. They infect various cell types with high efficiency, and their transient extrachromosomal state ensures safety without genomic integration. Adenovirus DNA undergoes transcription, allowing high-level gene expression in most mammalian cells. Creative Biogene offers a diverse premade adenovirus collection with full-length ORFs, tags, and reporters for comprehensive gene studies. If your gene of interest is not in our collection, our customized adenoviral service caters to specific project needs, exemplifying our leadership in adenoviral technology.

Advantages of Our Gene-Specific Recombinant Adenoviruses

  • Efficient Gene Delivery: Recombinant adenoviruses are highly efficient tools for delivering and expressing genes in mammalian cells.
  • Broad Cell Compatibility: Adenoviruses exhibit superior infectivity across a diverse range of cell types, suitable for both in vivo and in vitro applications.
  • Safe Non-integrating Technology: Adenovirus DNA remains in a transient extrachromosomal state, ensuring safety by avoiding integration into the host cell's genetic material.
  • High-Level Gene Expression: Recombinant adenoviruses enable robust gene expression and achieve high virus titers in various mammalian cells.
  • Premade Collection and Customization: Creative Biogene offers an extensive premade adenovirus collection, and our advanced technologies support customization for specific research needs.

The gene-specific adenoviral vectors enable simple and rapid functional studies by efficiently transducing target cells in vitro or in vivo to induce robust transient overexpression. The principle involves cloning full-length ORFs into adenoviral transfer vectors optimized for high gene delivery. By circumventing plasmid cloning and transfection, the ready-to-use adenoviruses accelerate experiments investigating exogenous gene overexpression phenotypes and downstream molecular impacts. We provide powerful customizable tools for diverse functional genomics applications：

  • Efficient Transduction: Achieve strong and transient gene overexpression through efficient transduction methods, ensuring robust experimental results.
  • Time-Efficient Functional Studies: Expedite functional studies by eliminating the need for time-consuming cloning and transfection processes, allowing researchers to focus on core aspects of their experiments.
  • Phenotypic and Signaling Impact: Investigate the influence of exogenous genes on phenotypes and signaling pathways, providing valuable insights into gene function and cellular responses.
  • Versatile Functional Genomics Tools: Serve as powerful tools for a wide range of functional genomics applications, offering versatility in studying gene functions across different biological contexts and systems.
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Over the past decade, thousands of non-coding RNAs (ncRNAs) have been observed. MicroRNAs (miRNAs) are a class of ncRNAs consisting of 18-24 nucleotides that are usually highly conserved among different species. Accumulating evidence indicates that miRNAs play important roles in various biological processes such as development, differentiation, and stemness by binding to the 5'UTR, ORF, or 3'UTR of target gene mRNAs to repress their translation or induce their degradation effect.

What Is Human miRNA?

Accumulating evidence indicates that miRNAs are involved in the pathogenesis of many diseases, such as cancer, diabetes, cardiovascular disease and neurodegenerative diseases. Currently, the miRNA repository miRBase lists 1917 human precursor miRNAs (pMIRs) and 2654 mature miRNAs (mMIRs), and it is estimated that more than 60% of human protein-coding genes contain predicted miRNA target sites. Many therapeutic miRNAs and small interfering RNAs (siRNAs) have important biopharmaceutical properties and have been commercially developed as potential medical treatments. Furthermore, the development of reagents that can strongly inhibit and overexpress specific miRNAs is of great interest and is important for basic miRNA research and as a possible therapeutic strategy.

Applications of Human miRNA Cell Line?

The use of human miRNA-overexpressing cell lines is critical for understanding the roles of microRNAs in various biological processes. miRNA-overexpressing cell lines have become an indispensable tool for studying the functional significance of miRNAs in various biological processes. Their ability to promote the stable overexpression of specific miRNAs facilitates the study of their effects on cellular processes. The use of miRNA-overexpressing cell lines has revealed important roles for miRNAs in cancer, neurology, stem cell differentiation, immune response, and cardiovascular disease. The continued development of miRNA-overexpressing cell lines promises to further elucidate the roles of miRNAs in physiology and pathology and may lead to the development of new therapeutic strategies for a range of diseases.

Our Human miRNA Overexpression Stable Cell Lines

miRNA overexpressing cell lines are usually constructed by transfecting a plasmid or viral vector containing the miRNA of interest into host cells. The transfection process promotes the integration of miRNAs into the host genome, resulting in stable overexpression of miRNAs. Creative Biogene is developing stable cell lines overexpressing human miRNA precursors covered in the current miRbase. Our human miRNA overexpression stable cell lines are constructed by lentivirus transduction or non-viral plasmid transfection of vectors optimized by our scientists to enable expression and maturation of miRNAs inside cells.

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Agomir/Antagomir is a chemically modified experimental tool for miRNA functional studies. IntegrateRNA utilizes our cutting-edge technology and state-of-the-art equipment to efficiently synthesize high-quality agomir and antagomir molecules, delivering carefully engineered, synthesized, and purified high-quality sequences to our customers. Our strong portfolio of expertise and technologies also allows us to provide additional services such as base modification, conjugation with various molecules such as fluorescent dyes or nanoparticles for imaging or targeting purposes, and even in vivo delivery of agonists or anti-agonists to study their biological effects.

Introduction of miRNA Agomir/Antagomir

Both miRNA agomir and antagomir are chemically modified microRNA molecules that have a wide range of applications due to their ability to manipulate miRNA expression. miRNA agomirs mimic the function of endogenous miRNAs. They are specially labeled and chemically modified small double-stranded RNA molecules that are complementary to the target miRNA sequence. Upon introduction into cells, agomirs bind to endogenous miRNAs and activate their function, thereby enhancing repression of target genes. miRNA antagomirs are chemically modified single-stranded small RNA molecules, but they are designed to specifically target and inhibit specific endogenous miRNAs based on the miRNA sequence. Antagomirs bind to the target miRNA sequence, preventing it from interacting with the mRNA target, which results in an increase in the expression of the target gene.

Our microRNA Agomir & Antagomir Synthesis Service

IntegrateRNA provides high-quality miRNA agomir/antagomir using our advanced fully automated synthesis and purification equipment, and we offer flexible customization, modification, and quality validation to meet any of our customers' research demands.

Our services mainly include the following:

  • Custom miRNA agomir/antagomir synthesis service

IntegrrateRNA provides flexible custom synthesis of miRNA agomir/antagomir by chemical synthesis method. Our scientists provide synthetic design services for specific targets or species and modify and purify them according to customers' research demands. We also utilize advanced miRNA sequencing platforms to fully test and validate the synthesized sequences to ensure the purity and integrity of miRNA agomir/antagomir molecules.

  • miRNA agomir/antagomir modifications service

We offer a variety of miRNA modification and labeling services that can modify synthetic miRNA agomir or antagomir to improve their stability or target specificity. 

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Focus on genome editing and circRNA fields for many years, IntegrateRNA provides a comprehensive solution for specific knockdown of circRNA without changes of expression level of their linear counterparts. Our one-stop solution including project consulting, sgRNA design, sgRNA construction, lentivirus/AAV packaging, cell transfection, circRNA expression evaluation and cell line generation. Besides, IntergrateRNA develops several integrated vector contains RfxCas13d, crRNA and GFP, to facilitate your circRNA functional research.

Circular RNAs (circRNAs) produced from back-spliced exons are widely expressed, but individual circRNA functions remain poorly understood due to inadequate methods, such as RNAi and genome engineering, in distinguishing overlapped exons in circRNAs from those in linear cognate mRNAs. Recent studies have established that RNA-targeting type VI CRISPR effectors, known as Cas13a, Cas13b and Cas13d RNases, can be directed to cleave single-stranded RNA targets carrying complementary protospacers by a CRISPR RNA in mammalian cells. It’s reported that RfxCas13d/BSJ-gRNA system can be applied in circRNA knockdown with higher specificity and lower off-target effects. Most importantly, RfxCas13d can accurately discriminates circRNAs from linear mRNAs. Thus, RfxCas13d/BSJ-gRNA system is a simple and scalable tool that can be used to identify functional circRNAs.

Workflow of circRNA Knockdown

  • sgRNA design, synthesis and construction
  • Virus Packaging
  • Cell transfection
  • Expression evaluation of circRNA and its host gene
  • Stable cell line generation

Benefits

  • Guaranteed circRNA knockdown
  • Lower off-target effects and lower cell toxicity
  • Affordable genome-scale circRNA screening tool
  • Active in vivo and in vitro, enabling the analysis RNA function in a wide range of model systems
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IntegrateRNA offers strong support in oligonucleotide drug discovery research from target validation, selection, and creation of oligonucleotide drug candidates to preclinical development. We have extensive experience in the discovery of mRNA-targeted drugs and in silico screening of ASO/siRNA. We use state-of-the-art technologies including bioinformatics, RNAi, high-throughput screening, along with profiling of target expression in relevant cell types which are available to help support your project.

Effectively identifying and validating therapeutic targets and biomarkers are key to effective precision therapeutic development. mRNAs currently the target of ASO and small interfering RNA (siRNA) drugs are increasingly being recognized as important drug targets that could eventually surpass the number of druggable proteins. Thus, the discovery of drugs that target mRNA is a promising direction for drug development and a new paradigm for the pharmaceutical industry.

Target Identification and Validation

  • Identify druggable mRNA targets associated with a disease of interest.
  • Confirm that mRNA targets are involved in disease etiology and result in a therapeutic benefit when altered.
  • Identify and optimize oligo molecules that have a pharmacological effect on the targets.

In Silico siRNA/ASO Screening

  • In silico oligonucleotide sequence design
    • Secondary structure prediction of the mRNA target.
    • Identification of preferable mRNA local secondary structures for ASO/siRNA binding.
    • Select the best predicted ASO/siRNA for docking and simulation.
    • Molecular dynamic simulation of the docked structure of mRNA-ASO/siRNA
  • In silico oligonucleotide modification/conjugation design
  • Efficiency evaluation of predicted ASO/siRNA
  • In silico off-target analysis